Gel stained with Coomassie Blue
(3/7/2012)
Last week I talked at length about gel electrophoresis and the prep process using green fluorescent protein (GFP) in my entry Day 55: Gel electrophoresis. As I mentioned, Coomassie Brilliant Blue is the most common protein stain and one of the two stains we used after running the gels last week. In addition to the gel above, stained with Coomassie Blue, we also used a silver stain. Silver staining is incredibly sensitive. When transferring the gel, you must take care in handling the gel because even fingerprints can leave behind protein remnants that will stain.
Silver staining
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